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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in several tumors, which leads to chromosome instability, and so might contribute to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital part in selling mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already explained. Two additional splice variants were being reported but in depth nucleotide sequence information and facts isn't nonetheless out there.
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Blocking peptides are peptides that bind specifically into the goal antibody and block antibody binding. These peptide commonly includes the epitope recognized from the antibody.
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This tutorial outlines additional achievable optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...
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This guidebook outlines further more achievable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
Antibodies bound to the blocking peptide not bind for the epitope about the goal protein. By evaluating the staining from your blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs to your hugely conserved cyclin family, whose users are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates on the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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